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Dark‐inducible genes from Arabidopsis thaliana are associated with leaf senescence and repressed by sugars
Author(s) -
Fujiki Yuki,
Yoshikawa Yoko,
Sato Tokuyuki,
Inada Noriko,
Ito Masaki,
Nishida Ikuo,
Watanabe Akira
Publication year - 2001
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2001.1110312.x
Subject(s) - asparagine synthetase , arabidopsis thaliana , biology , gene , arabidopsis , asparagine , gene expression , complementary dna , photosynthesis , biochemistry , sucrose , sugar , enzyme , mutant
We have isolated 5 cDNA clones ( din2, din6 , din9, din10 and din11 ) corresponding to genes, the transcripts of which accumulated in leaves of Arabidopsis thaliana kept in the dark. These cDNA clones encode proteins similar to β ‐glucosidase (EC 3.2.1.21, din2 ), asparagine synthetase (EC 6.3.5.4, din6 ), phosphomannose isomerase (EC 5.3.1.8, din9 ), seed imbibition protein ( din10) and 2‐oxoacid‐dependent dioxygenases ( din11 ). Accumulation of the transcripts from din 6 and din10 occurred within 3 h after plants were transferred to darkness. The transcripts from din2, din9 and din11 were only detected after 24 h of dark treatment. We also observed the accumulation of the din transcripts in senescing leaves. Application of a photosynthesis inhibitor, 3‐(3,4‐dichlorophenyl)‐1‐1‐dimethyl‐urea, induced the expression of the din genes under illumination. Application of sucrose to detached leaves suppressed the accumulation of the din transcripts in the dark. These results indicate that expression of these genes partly depends on cellular sugar level. The sugar‐modulated expression of the din genes suggests that dark‐induced expression of these genes might be related to sugar starvation occurring in leaf cells in the dark, when the photosynthesis is hindered.