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Nitrate reduction in tendrils of semi‐leafless pea
Author(s) -
González Esther M.,
Cabrerizo Pablo M.,
Royuela Mercedes,
AparicioTejo Pedro M.,
ArreseIgor Cesar
Publication year - 2001
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2001.1110310.x
Subject(s) - tendril , pisum , nitrate reductase , nitrate , sativum , biology , botany , ecology
The recessive gene af produces a modification of Pisum sativum L. leaf morphology, where leaflets are replaced by tendrils. Previous reports have suggested that tendrils may contribute to plant growth in a similar way to flat leaf structures, but these reports have been restricted to carbon metabolism. In this work, we investigate the nitrate reduction (nitrate reductase activity) capacity of tendrils of a semi‐leafless variety, Solara, in relation to other leaf structures. Maximum nitrate reductase activity (EC 1.6.6.1), expressed on a protein basis, was significantly lower in tendrils compared to flat structures. However, the activation state of nitrate reductase was significantly higher in tendrils, reaching 70%, compared to flat leaf structures. According to these results, tendrils contributed up to 25% of the overall plant nitrate reduction in the semi‐leafless variety. This figure was even higher when nitrate reduction was calculated from in vivo measurements. The results are discussed in relation to nitrate, magnesium, carbohydrates, amino acids and adenylate levels of tendrils.