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Two γ ‐tubulin isoforms are differentially expressed during development in Helianthus annuus
Author(s) -
Petitprez Michel,
Caumont Catherine,
Barthou Henri,
Wright Michel,
Alibert Gilbert
Publication year - 2001
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2001.1110113.x
Subject(s) - tubulin , cytoskeleton , microtubule , biology , helianthus annuus , microbiology and biotechnology , microtubule organizing center , microtubule nucleation , gene isoform , centrosome , somatic cell , microtubule associated protein , botany , sunflower , cell , genetics , cell cycle , gene , agronomy
The cytoskeleton is involved in major developmental events in plant cell growth and differentiation. Nucleation events play a key role in the dynamic and organization of the microtubule (Mt) cytoskeleton. Among many proteins involved in Mt nucleation, γ ‐tubulin has been identified as an essential component of the Mt organizing centers (MTOC). In protoplasts, somatic embryogenesis induction has been correlated with remodeling of Mt cytoskeleton. We have investigated the specific developmental expression of γ ‐tubulin in Helianthus annuus . Two γ ‐tubulin isoforms have been detected by immunoblotting, with bands at 52 and 58 kDa. The larger γ ‐tubulin (58 kDa) is present in all the sunflower tissues tested and is associated with the nucleus. The smaller γ ‐tubulin (52 kDa), differing from the former at the carboxy‐terminal end, is only present in meristematic and dedifferentiated cells and is not bound to the nucleus. This first demonstration of the presence of two γ ‐tubulins in plant cells is discussed in terms of distinct roles in the nucleation and organization of Mts.