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A novel method for growing Arabidopsis thaliana plants hydroponically
Author(s) -
Arteca Richard N.,
Arteca Jeannette M.
Publication year - 2000
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2000.108002188.x
Subject(s) - arabidopsis , arabidopsis thaliana , hydroponics , biology , botany , nutrient , horticulture , ecology , biochemistry , gene , mutant
In this report we describe the development of a simple system for growing individual Arabidopsis plants hydroponically under environmental conditions which are simple to maintain while maximizing growth and development. The system consists of a Life Raft Float Unit with a 14–20 mm sponge inserted into its center; this unit is placed into a Magenta GA7 vessel containing Arabidopsis nutrient solution. Plants grown in this experimental system do not require aeration, 125 μmol m −2 s −1 supports vigorous plant growth, 1/4 to 1/8 strength Arabidopsis nutrient solution promotes maximal growth while higher or lower levels have adverse effects and temperatures between 18 and 24°C were found to be optimal. Plants grown under these conditions exhibit physiological factors such as time to flowering and overall appearance similar to those grown in potting media. The low maintenance method described in this paper provides an alternative to existing hydroponic methods used to grow Arabidopsis . Adaptation of this system for growing large numbers of plants in 1 container is also discussed.