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Arabinogalactan proteins in embryogenic and non‐embryogenic callus cultures of Euphorbia pulcherrima
Author(s) -
SaareSurminski Katja,
Preil Walter,
Knox J. Paul,
Lieberei Reinhard
Publication year - 2000
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2000.108002180.x
Subject(s) - callus , somatic embryogenesis , arabinogalactan , euphorbia , biology , botany , tissue culture , somatic cell , in vitro , biochemistry , cell wall , gene
The arabinogalactan protein (AGP) fractions of embryogenic and non‐embryogenic callus lines of Euphorbia pulcherrima Willd. ex. Klotzsch were analysed over a cultivation period of 9 weeks using the β ‐glucosyl Yariv reagent and an anti‐AGP antibody (LM2). The amount of AGPs detected with the Yariv reagent increased in embryogenic cultures during the development of somatic embryos. The embryogenic and non‐embryogenic callus contained different sets of AGPs characterized with the Yariv reagent and the LM2 monoclonal antibody. AGPs recognized by LM2 are localized primarily in the protodermal cells of globular somatic embryos. The development of somatic embryos of E. pulcherrima appears to be associated with the presence of particular AGPs.