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Antioxidant status of anoxia‐tolerant and ‐intolerant plant species under anoxia and reaeration
Author(s) -
Blokhina Olga B.,
Virolainen Eija,
Fagerstedt Kurt V.,
Hoikkala Antti,
Wähälä Kristiina,
Chirkova Tamara V.
Publication year - 2000
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2000.100405.x
Subject(s) - ascorbic acid , antioxidant , glutathione , dehydroascorbic acid , oryza sativa , botany , reactive oxygen species , biology , food science , biochemistry , chemistry , horticulture , enzyme , gene
The redox potential of the cell, as well as the antioxidant status of the tissue, are considered to be important regulatory constituents in an adaptive response in plants. Here the involvement of active antioxidants ascorbic acid (AA), reduced glutathione (GSH) and α ‐ and β ‐tocopherols in reactive oxygen species scavenging, and the effect of anoxic stress on their reduction state were studied in 4 anoxia‐tolerant and ‐intolerant plant species: Iris germanica L., Iris pseudacorus L., wheat ( Triticum aestivum L. cv. Leningradka) and rice ( Oryza sativa L. cv. VNIIR). The initial antioxidant content (both AA and GSH) was higher in the rhizomes of the more anoxia‐tolerant Iris spp., as compared with that of the roots of the cereals. The predominant form of ascorbate was dehydroascorbic acid (DHA) in the cereals and AA in the Iris spp. Imposition of anoxia with subsequent reoxygenation resulted in an overall depletion of the reduced forms of antioxidants. No concurrent increase in oxidised forms (DHA and conjugated glutathione) was observed in anoxic samples. α ‐tocopherol content in Iris spp. was in the range 1–2 μg g −1 fresh weight, while β ‐tocopherol content was higher in the anoxia‐intolerant I. germanica (7.2 μg g −1 fresh weight) as compared with the tolerant I. pseudacorus (1.5 μg g −1 fresh weight). In I. pseudacorus , a significant decrease in α ‐ and β ‐tocopherol levels was observed only after long‐term (45 days) anoxia. The results suggested exclusion of AA and GSH from the redox cycling under prolonged anoxia, and a concomitant decrease in the redox state, as well as an anoxia‐induced depletion of α ‐ and β ‐tocopherols.