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Effect of Al on the phytosiderophore‐mediated solubilization of Fe and uptake of Fe‐phytosiderophore complex in wheat ( Triticum aestivum )
Author(s) -
Chang YiChieh,
Ma Jian Feng,
Iwashita Takashi,
Matsumoto Hideaki
Publication year - 1999
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.1999.106109.x
Subject(s) - elongation , solubilization , absorption (acoustics) , chemistry , nuclear chemistry , complex formation , biophysics , biochemistry , biology , materials science , inorganic chemistry , metallurgy , composite material , ultimate tensile strength
The effect of Aluminum (Al) on phytosiderophore‐mediated solubilization of insoluble Fe and the uptake of phytosiderophore‐Fe 3+ complex was examined in wheat ( Triticum aestivum L. cv. Atlas 66). Al addition did not affect the Fe solubilization by 2′‐deoxymugineic acid (DMA), although Cu addition significantly inhibited the solubilization capacity. Addition of ten times more Al than Fe to the solution of DMA‐Fe 3+ complex did not decrease the absorption of the DMA‐Fe 3+ complex at 375 nm. Furthermore, NMR study indicated that Al did not shift the proton chemical shifts of DMA. All these results suggest that Al could not form a complex with the phytosiderophore, and is thereby unlikely to affect the process of phytosiderophore‐mediated solubilization of Fe. Exposure of root to Al up to 100 μ M for 3 h did not inhibit the DMA‐Fe 3+ uptake by the roots, but longer pretreatment (>6 h) inhibited the uptake of the DMA‐Fe 3+ by more than 50%. Neither the uptake of DMA‐Fe 3+ nor root elongation was inhibited by 24 h pretreatment with 10 μ M Al, but both uptake and root elongation were inhibited by higher Al (>20 μ M ) pretreatment. These results suggest that Al did not directly block the transport of the phytosiderophore‐Fe 3+ complex, and that the decreased uptake of the phytosiderophore‐Fe 3+ complex resulted from the roots being damaged by Al.