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Inducible expression of plasma membrane H + ‐ATPase in the roots of figleaf gourd plants under chilling root temperature
Author(s) -
Ahn Sung Ju,
Im Yang Ju,
Chung Gap Chae,
Cho Baik Ho
Publication year - 1999
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.1999.106105.x
Subject(s) - gourd , chemistry , cycloheximide , atpase , phytotron , horticulture , membrane , botany , biochemistry , biology , enzyme , protein biosynthesis
Figleaf gourd plants were grown under different root temperatures and the acclimation process of the roots was investigated. Conditioning of the roots by a gradual decrease (2°C h −1 ) in root temperature to 6°C considerably lowered the electrolyte leakage of the plant leaves compared to non‐conditioned roots. A root temperature of 6°C was critical for the increase in H + ‐ATPase (EC 3.6.1.35) activity in the plasma membranes of the roots and this increase was abolished by the addition of cycloheximide in the root medium. The H + ‐transport rate of the plasma membranes was also increased by low root temperature. Increases in the amount of the plasma membrane H + ‐ATPase gene transcription and translation in the plant roots under chilling root temperature were demonstrated by Northern and Western blot analysis, respectively. A correlation between the leaf temperature, measured by an infra‐red thermometer, and the H + ‐ATPase activity suggests the involvement of H + ‐ATPase in the root acclimation process.