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Differential expression of DAHP synthase and chorismate mutase in various organs of Brassica juncea and the effect of external factors on enzyme activity
Author(s) -
Sharma Ranjana,
Jain Mukesh,
Bhatnagar Raj K.,
BhallaSarin Neera
Publication year - 1999
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.1999.105419.x
Subject(s) - chorismate mutase , brassica , enzyme , biochemistry , biology , phosphoglycerate mutase , mutase , botany , biosynthesis , glycolysis
The multibranched shikimic acid pathway was discovered as the biosynthetic route to the aromatic amino acids phenylalanine, tyrosine and tryptophan and a host of other secondary metabolites. An extensive body of work is available on the characterization of various enzymes of this pathway in order to understand the underlying mechanisms of aromatic amino acid biosynthesis and secondary metabolism in higher plants. In the present investigation, selective assays, based on feedback regulation patterns and divalent cation requirements, were used to monitor the isozyme profiles of two of the key regulatory enzymes of this pathway. 3‐Deoxy‐ d ‐arabino heptulosonate‐7‐phosphate synthase (DAHP synthase/DS) (EC 4.1.2.15) and chorismate mutase (CM) (EC 5.4.99.4) have been characterized from different vegetative and reproductive organs of Brassica juncea cv. Pusa Bold. An attempt has also been made to investigate the effect of external factors, such as light and wounding on the regulation of these enzymes. The results reveal differential expression of DAHP synthase and CM in various organs of Brassica and an adaptability of plants to various stresses by up or down regulation of these enzymes.