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Protective effect of glutathione on the cytotoxicity caused by a combination of aluminum and iron in suspension‐cultured tobacco cells
Author(s) -
Yamaguchi Yukiko,
Yamamoto Yoko,
Ikegawa Hiroshi,
Matsumoto Hideaki
Publication year - 1999
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.1999.105305.x
Subject(s) - glutathione , buthionine sulfoximine , toxicity , chemistry , biochemistry , lipid peroxidation , viability assay , intracellular , cytotoxicity , antioxidant , in vitro , enzyme , organic chemistry
The role of endogenous glutathione (GSH) in the protection of suspension‐cultured tobacco cells from aluminum (Al) toxicity was examined. Cells at the logarithmic phase of growth were treated with or without Al in nutrient medium prepared without P i and EDTA. In the absence of Al, total GSH content (including oxidized glutathione [GSSG]) increased gradually. In the presence of Al, the increase of GSH was repressed. This effect was observed before the loss of plasma membrane integrity and the loss of cell viability. In contrast, GSSG content in cells increased in the presence of Al. GSH‐deprived cells were prepared by culturing cells with buthionine sulfoximine (an inhibitor of Γ ‐glutamylcysteine synthetase) for 24 h. Total GSH content in GSH‐deprived cells was 6% of that in normal cells. The GSH‐deprived cells exhibited a higher degree of lipid peroxidation, increased accumulation of Al, and greater loss of viability than normal cells. These results suggest that GSH protects cells from the oxidative membrane damage caused by a combination of Al and Fe(II) possibly by both direct consumption of GSH and oxidation of GSH.