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Hormonal cross‐talk regulates the Arabidopsis thaliana 1‐aminocyclopropane‐1‐carboxylate synthase gene 1 in a developmental and tissue‐dependent manner
Author(s) -
RodriguesPousada Renato,
Van Caeneghem Wim,
Chauvaux Nancy,
Van Onckelen Harry,
Van Montagu Marc,
Van Der Straeten Dominique
Publication year - 1999
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.1999.105217.x
Subject(s) - auxin , biology , arabidopsis thaliana , cytokinin , mutant , hormone , arabidopsis , gene expression , biochemistry , endogeny , ethylene , gene , organogenesis , transgene , enzyme , microbiology and biotechnology , catalysis
The Arabidopsis thaliana (L.) Heynh. 1‐aminocyclopropane‐1‐carboxylate synthase gene 1 ( AT‐ACS1 ) was found to be developmentally regulated with pronounced activity in immature tissues. The effect of different hormones on this expression pattern was studied by using a transgenic line carrying the β ‐glucuronidase ( GUS ) gene under control of the AT‐ACS1 promoter. In addition, the level of mRNA for AT‐ACS1 was assayed in different ethylene and auxin‐insensitive backgrounds, as well as in an auxin homeostasis mutant by using competitive reverse transcriptase polymerase chain reaction (RT‐PCR). The results support the existence of a hormonal cross‐talk involving auxin, cytokinin, and ethylene in the regulation of AT‐ACS1 . These hormonal controls act in a developmental and tissue‐dependent manner.