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Insulin‐stimulated ribosomal protein synthesis in maize embryonic axes during germination
Author(s) -
Sánchez de Jiménez Estela,
BeltránPeña Elda,
OrtízLópez Adriana
Publication year - 1999
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.1999.105122.x
Subject(s) - wortmannin , ribosomal protein , insulin , polysome , signal transduction , phosphorylation , ribosomal rna , biology , eukaryotic small ribosomal subunit , protein biosynthesis , translation (biology) , ribosome , kinase , microbiology and biotechnology , biochemistry , messenger rna , phosphatidylinositol , endocrinology , gene , rna
Addition of insulin to maize seed ( Zea mays L. cv. Chalqueño) was found to accelerate germination and seedling growth. Insulin‐stimulated maize axes showed enhancement of S‐methionine incorporation into ribosomal proteins (rp) and mobilization of S 6 rp mRNA into polysomes. Increase in S 6 rp phosphorylation of the small ribosomal subunit (40S) was observed in P‐orthophosphate pulse‐labeled experiments when maize axes were stimulated by insulin. Application of either wortmannin or rapamycin, inhibitors of protein kinases of the insulin transduction pathway, abolished the insulin stimulatory effect on S 6 rp phosphorylation and on ribosomal protein synthesis. The above data are interpreted as an indication of the existence of an insulin‐stimulated signal transduction pathway in maize tissues that is involved in the regulation of translation.