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Cultivar variability in the Agrobacterium ‐rice cell interaction and plant regeneration
Author(s) -
Lee SungHo,
Shon YoungGoel,
Lee Soo In,
Kim Cha Young,
Koo Ja Choon,
Lim Chae Oh,
Choi Young Ju,
Han Changdeok,
Chung Chang Ho,
Ryong Choe Zhin,
Cho Moo Je
Publication year - 1999
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.1999.100311.x
Subject(s) - agrobacterium tumefaciens , biology , cultivar , oryza sativa , agrobacterium , regeneration (biology) , transformation (genetics) , shoot , kanamycin , botany , gus reporter system , horticulture , gene , genetics
Thirteen cultivars of rice ( Oryza sativa ) were tested for plant regeneration from calli initiated from the scutella of mature seeds by water stress treatment using a high concentration of agarose, and examined for their response to Agrobacterium tumefaciens LBA4404, carrying a plasmid pTOK233, harboring genes for kanamycin resistance ( npt II), hygromycin resistance ( hpt ) and β ‐glucuronidase ( gus ). Plant regeneration frequency was considerably increased in most of the cultivars when the calli were treated with water stress, as compared with untreated controls. In particular, the cultivars Dongjinbyeo, IR43, Nagdongbyeo and Sinseonchalbyeo showed an increased frequency of shoot regeneration. Expression of GUS was detected in all of the co‐cultivated cultivars. Based on GUS expression at 3 days after co‐cultivation with A. tumefaciens , three rice cultivars (Dongjinbyeo, Hwayoungbyeo and Nagdongbyeo) were judged highly susceptible to A. tumefaciens , while Milyang 23, Nonganbyeo and Samgangbyeo cultivars were weakly susceptible. Plantlets were readily regenerated when the hygromycin‐resistant calli were transferred to a regeneration medium containing hygromycin. Intense blue staining was observed in GUS assays of leaf segments, roots and flower organs from regenerated plants. Stable integration and expression of the introduced hpt and gus genes were confirmed by Southern blot analysis of the transformants. Therefore, Dongjinbyeo and Nagdongbyeo cultivars proved to be both highly susceptible to A. tumefaciens and highly responsive to plant regeneration.