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Cell wall modifications in bean ( Phaseolus vulgaris ) callus cultures tolerant to isoxaben
Author(s) -
DíazCacho Pilar,
Moral Rosa,
Encina Antonio,
Luis Acebes José,
Alvarez Jesús
Publication year - 1999
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.1999.100108.x
Subject(s) - phaseolus , callus , uronic acid , cell wall , botany , biology , cellulose , chemistry , biochemistry , polysaccharide
Cultures of bean ( Phaseolus vulgaris L.) callus were adapted for growth in a 12 μ M concentration of the herbicide isoxaben; 1 200‐fold greater than the I 50 calculated. Tolerant calluses grew slower, were browner and harder than nontolerant ones and formed large globular corpuscles on their surface. Cell wall fractionation of tolerant cells indicated that the mechanism of adaptation depends on the ability of these cells to divide and expand with a modified wall. The walls of tolerant cells growing on isoxaben differ from those of nontolerant cells by having reduced levels of cellulose and hemicelluloses and a higher uronic acid content. These differences are already found in adaptations to low concentrations of isoxaben and keep to the highest adaptation levels tested without any great change.