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Tolerance to atmospheric ozone in transgenic tobacco over‐expressing glutathione synthetase in plastids
Author(s) -
Wellburn Florence A. M.,
Creissen Gary P.,
Lake Janice A.,
Mullineaux Phil M.,
Wellburn Alan R.
Publication year - 1998
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.1998.1040415.x
Subject(s) - plastid , glutathione , transgene , ozone , glutathione synthetase , nicotiana tabacum , biology , botany , chemistry , biochemistry , gene , chloroplast , enzyme , organic chemistry
A cross between transgenic tobacco ( Nicotiana tabacum L.) plants which over‐expressed either γ‐glutamylcysteine synthetase (cpGSHI) or glutathione synthetase (cpGSHII) in their chloroplasts was used to compare the consequences of over‐expression of components of the glutathione synthetic pathway on tolerance to atmospheric O 3 or paraquat. A high proportion (50%) of those progeny which carried the cpGSHII transgene alone showed tolerance to atmospheric O 3 but not to paraquat. Progeny of an additional two, independent, self‐pollinated primary transgenic lines, which segregated in a Mendelian fashion for the presence of the cpGSHII transgene and therefore included both transformed and non‐transformed (recessive, wild‐type) plants, were also challenged by fumigation with O 3 . Again, in both cases, about 50% of the plants expressing the epGSHII transgene were found to be O 3 ‐tolerant on the basis of reduced ethylene emissions and increased or unchanged total pigment concentrations. However, this tolerance was not due to specific changes in stomatal densities.