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Cell death caused by a combination of aluminum and iron in cultured tobacco cells
Author(s) -
Ikegawa Hiroshi,
Yamamoto Yoko,
Matsumoto Hideaki
Publication year - 1998
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.1998.1040324.x
Subject(s) - evans blue , programmed cell death , nicotiana tabacum , cleavage (geology) , cell growth , chemistry , staining , membrane integrity , mtt assay , neutral red , growth inhibition , membrane , cell , microbiology and biotechnology , apoptosis , biochemistry , biology , in vitro , cytotoxicity , endocrinology , paleontology , genetics , fracture (geology) , gene
The inhibition of growth of tobacco cells ( Nicotiana tabacum L. cv. Samsun) after treatment with A1 in medium containing high concentrations of cations requires the presence of Fe (II or III) during the treatment. We examined whether the inhibition of the post‐treatment growth is due to cell death occurring during the treatment with A1 and Fe. In cells at the end of A1 treatment, the integrity of the plasma membrane and the integrity of the mitochondrial inner membrane were monitored by use of Evans blue staining and the cleavage of 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT), respectively. Time‐course and dose‐response experiments indicate that the inhibition of post‐treatment growth is strongly related to Evans blue uptake, but not to MTT cleavage. These results suggest that the loss of integrity of the plasma membrane caused by a combination of Al and Fe directly contributes to cell death and the inhibition of post‐treatment growth.