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Elongation factor 1α (EF‐1α) transcript levels are developmentally and environmentally regulated in apple plants
Author(s) -
Watillon Bernard,
Kettmann Richard,
Boxus Philippe,
Burny Arsène
Publication year - 1998
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.1998.1040101.x
Subject(s) - elongation , eukaryotic translation elongation factor 1 alpha 1 , biology , elongation factor , microbiology and biotechnology , botany , gene , genetics , rna , ribosome , materials science , metallurgy , ultimate tensile strength
A cDNA fragment corresponding to an elongation factor lα subunit (EF‐lα) messenger was amplified and used to isolate a corresponding full‐length copy from an apple ( Malus domestica [L.] Borkh) cv. McIntosh ‘Wijcik’ cDNA library. Genomic Southern blot analysis using this probe confirmed that the EF‐1α factor is encoded by a multigenic family in apple. Northern blot analysis of corresponding transcript levels in apple tree organs at various developmental stages revealed that very young leaves contain higher levels of EF‐1α mRNA than older leaves, although significant levels could be detected in all organs tested. In response to wounding, a transient decrease in EF‐1α mRNA levels was observed in apple plantlets, followed by a slight increase. A 24‐h incubation at low temperature (5°C) induced a significant decrease in corresponding transcript abundance. In contrast, when dark‐grown, etiolated, apple plantlets were transferred to light conditions and analyzed with the same probe, few modifications in EF‐1α transcript abundance were detected.