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Transient freezing regulates expression of extensin‐type genes in winter oilseed rape
Author(s) -
Kozbial Piotr Z.,
Jerzmanowski Andrzej,
Shirsat Anil H.,
Kacperska Alina
Publication year - 1998
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.1998.1030214.x
Subject(s) - frost (temperature) , gene expression , biology , brassica , incubation , messenger rna , cell wall , gene , botany , microbiology and biotechnology , horticulture , biochemistry , geomorphology , geology
In order to identify the molecular mechanisms underlying the process of cold acclimation in plants, we examined the expression of extensin‐type genes in Brassica napas L. var. oleifera L. leaves. subjected to various low temperature treatments. Northern analysis showed that two transcripts of 1 260 nt and 1 450 nt, strongly hybridized to the extA probe and accumulated to high levels in leaves subjected to transient freezing treatments, giving rise to ca 20% membrane injury (2 h at −3°C for non‐acclimated or at −12°C for cold‐acclimated leaves). The accumulation of the frost‐induced extensin mRNAs (especially of the 1 450‐nt transcript) was markedly higher than that seen upon mechanical wounding. Frost‐dependent induction of extensin mRNAs was markedly elevated both in extent and duration in cold‐acclimated as compared to non‐acclimated leaves. Post‐incubation of cold‐acclimated and frost‐treated leaves on ice instead of at room temperature prolonged the time course of extensin mRNA accumulation and resulted in a marked increase of the level of the transcripts in the material studied. Factors possibly involved in the induction of extensin gene transcription are discussed, as well as the role of extensin cell wall proteins in plant responses to freezing.