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Induction of an ACC synthase cDNA by ozone in light‐grown Arabidopsis thaliana leaves
Author(s) -
Vahala Jorma,
Schlagnhaufer Carl D.,
Pell Eva J.
Publication year - 1998
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.1998.1030106.x
Subject(s) - atp synthase , arabidopsis thaliana , ethylene , complementary dna , biology , biosynthesis , enzyme , gene , microbiology and biotechnology , biochemistry , chemistry , mutant , catalysis
The rate‐limiting enzyme in the ethylene biosynthesis pathway is 1‐aminocyclo‐propane‐1‐carboxylate (ACC) synthase. We have investigated an ozone (O 3 )‐induced ACC synthase gene in Arabidopsis thaliana . Four previously reported ACC synthase genes from A. thaliana ( ACS1, ACS2, ACS4 and ACS5 ) were not induced in response to O 3 stress. An O 3 ‐induced 1 098‐bp cDNA encoding ACC synthase was isolated from foliage of A. thaliana; this sequence was identical to that of ACS6 which could be induced by multiple stimuli including touch. When plants were treated with 350 nl l −1 of O 3 for 2 or 6 h. the evolution of ethylene became maximal after 1.5 h and gradually decreased thereafter. Similarly, the mRNA transcript of O 3 ‐induced ACC synthase was detected after 30 min of exposure, attained a maximum level after 1 h, and then declined. Visible damage in O 3 ‐treated plants was observed at the end of the 2‐h exposure. This ACC synthase gene in A. thaliana provides a useful tool for studying O 3 ‐induced responses related to ethylene production at the molecular and genetic levels.