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Mechanisms of Al‐induced iron chlorosis in wheat ( Triticum aestivum ). Al‐inhibited biosynthesis and secretion of phytosiderophore
Author(s) -
Chang YiChieh,
Ma Jian Feng,
Matsumoto Hideaki
Publication year - 1998
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.1998.1020102.x
Subject(s) - chlorosis , secretion , biosynthesis , ferric , cultivar , chemistry , biology , botany , nuclear chemistry , biochemistry , horticulture , inorganic chemistry , gene
Although Al‐induced iron chlorosis has been observed in many plants, the mechanisms responsible for this phenomenon are yet to be understood. We investigated the effect of Al on iron acquisition in a Strategy II plant, wheat ( Triticum aestivum L.) using both Al‐tolerant (Atlas 66) and ‐sensitive (Scout 66) cultivars. When iron was supplied as insoluble iron, ferric hydroxide, in the culture solution, both cultivars without Al treatment grew normally, while those with 100 µ M AlCl 3 developed chlorosis of the young leaves after 3 days of the treatment. A 21‐h treatment with 100 µ M AlCl 3 in 0.5 m M CaCl 2 solution (pH 4.5) decreased the amount of 2′‐deoxymugineic acid (DMA) secreted by Fe‐deficient Atlas 66 and Scout 66 plants by 85 and 90%, respectively. The amount of DMA secreted decreased with increasing external Al concentrations. Al treatment during the biosynthesis process caused the inhibition of that of DMA within 3 h. The secretion process was also found to be inhibited by Al, resulting in the biosynthesized DMA remaining in the roots. These results demonstrate the inhibition by Al of both biosynthesis and secretion of DMA attributed to Al‐induced iron chlorosis.