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Meta ‐topolin, an alternative to benzyladenine in tissue culture?
Author(s) -
Werbrouck Stefaan P. O.,
Strnad Miroslav,
Van Onckelen Henry A.,
Debergh Pierre C.
Publication year - 1996
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.1996.980210.x
Subject(s) - cytokinin , acclimatization , shoot , botany , in vitro , chemistry , glucoside , biology , auxin , biochemistry , medicine , alternative medicine , pathology , gene
The metabolism and the in vitro effects of the cytokinin N 6 ‐(3‐hydroxybenzyl)adenine ( meta‐topolin , mT) were compared with those of N 6 ‐benzyladenine (BA), N 6 ‐benzyl‐9‐(2‐tetrahydropyranyl)adenine (BPA) and N 6 ‐benzyladenine‐9‐riboside ([9R]BA) in micropropagated Spathiphyllum floribundum Schott cv. Petite. In vitro, BA, BPA and [9R]BA were mainly converted into the stable derivative N 6 ‐benzyladenine‐9‐glucoside ([9G]BA), located at the basal part of the plant. N 6 ‐(3‐O‐β‐D‐glucopyranosyl)benzyladenine‐9‐riboside {(OG)[9R]mT} was the main derivative of mT. This new cytokinin‐O‐glucoside, which was present in all plant parts, was metabolized much faster than [9G]BA during acclimatization. The effect of BA and mT on in vitro shoot and root production and post vitro rooting was compared. Only mT combined a good shoot production with in vitro root formation. The plants that developed on medium with 10 µ M or more mT rooted better during acclimatization than those developed on medium with equimolar concentrations of BA.