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The induction of freezing tolerance in jack pine seedlings: The role of root plasma membrane H + ‐ ATPase and redox activities
Author(s) -
Zhao Sheyun,
Colombo Stephen J.,
Blumwald Eduardo
Publication year - 1995
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.1995.930109.x
Subject(s) - conditioning , seedling , atpase , horticulture , biology , enzyme , botany , chemistry , biochemistry , statistics , mathematics
The acquired freezing tolerance of jack pine seedlings ( Pinus banksiana Lamb.) conditioned at low nonfreezing temperatures and short photoperiods was determined by comparison of seedling survival to that of nonconditioned (control) seedlings following exposure to −5 and −10°C. Compared to that of controls, survival of conditioned seedlings was markedly increased following exposure to freezing temperatures. A 1‐week conditioning treatment significantly increased the survival of the seedlings after exposure to −5°C, but was less effective on seedlings exposed to −10°C. Conditioning periods of 2 and 4 weeks resulted in higher survival of seedlings exposed to both −5 and −10°C. The changes of two root‐plasma‐membrane‐associated enzyme activities, H + ‐ATPase and NADH‐dependent ferricyanide reductase, were studied in enriched plasma membrane fractions during conditioning and after freezing. Post‐freezing activities of both enzymes were enhanced by conditioning at low temperatures and short photoperiods. These changes may be related to the increased frost hardiness also induced by conditioning.