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Method of birth alters interferon‐gamma and interleukin‐12 production by cord blood mononuclear cells
Author(s) -
Brown Mark A.,
Rad Parmis Y.,
Halonen Marilyn J.
Publication year - 2003
Publication title -
pediatric allergy and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.269
H-Index - 89
eISSN - 1399-3038
pISSN - 0905-6157
DOI - 10.1034/j.1399-3038.2003.00015.x
Subject(s) - peripheral blood mononuclear cell , medicine , cord blood , immunology , lipopolysaccharide , concanavalin a , umbilical cord , interleukin , interferon gamma , cytokine , andrology , biology , in vitro , biochemistry
Many uncertainties exist regarding the capability of cord blood mononuclear cells (CBMC) to produce cytokines. A number of conflicting reports led us to examine the effects of method of birth on CBMC production of interferon‐gamma (IFN‐γ), interleukin‐4 (IL‐4) and interleukin‐12 (IL‐12). While constitutive production of IL‐4 was found in both vaginally and cesarean‐delivered infants, constitutive IFN‐γ or IL‐12 production was found in neither. CBMC from vaginally delivered infants responded to stimulation with concanavalin A/phorbol 12‐myristate 13‐acetate (Con A/PMA), phytohemagglutinin (PHA), and lipopolysaccharide (LPS) with significantly higher levels of IFN‐γ than CBMC from unlabored cesarean section (CS) infants. Production of IL‐12 was increased in the vaginally delivered group in response to LPS and PHA but not to ConA/PMA. In contrast, mode of delivery was not associated with differences in IL‐4 production. These results indicate that mode of delivery significantly alters the capability of CBMC to produce some cytokines and therefore should be taken into account in interpreting fetal/neonatal mononuclear cell function studies.