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Interleukin‐15 enhances CD4 + CD45RA + expression on umbilical cord blood mononuclear cells
Author(s) -
Lin SyhJae,
Chao HsunChin
Publication year - 2001
Publication title -
pediatric allergy and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.269
H-Index - 89
eISSN - 1399-3038
pISSN - 0905-6157
DOI - 10.1034/j.1399-3038.2001.012004188.x
Subject(s) - umbilical cord , peripheral blood mononuclear cell , cord blood , medicine , cd8 , immunology , apoptosis , cytokine , andrology , microbiology and biotechnology , endocrinology , immune system , in vitro , biology , biochemistry
The reduced incidence of graft‐vs.‐host disease following umbilical cord blood (CB) transplantation may be related to the functional immaturity of newborn T cells expressing mainly the naive CD45RA phenotype. Expansion of CD4 + CD45RA + T cells using cytokines may benefit neonates and infants with human immunodeficiency virus (HIV) infection, as a preferential decline in CD4 + CD45RA + cells has been noted as HIV disease progresses. The aim of the study was to investigate the effect of interleukin (IL)‐15, a novel cytokine similar to IL‐2 in biological activities, on CD45RA/RO expression and apoptosis in umbilical cord blood (CB) and adult peripheral blood (APB) mononuclear cells (MNCs). Prior to culture, CB MNCs contained a greater number of CD4 + CD45RA + cells and fewer CD4 + CD45RO + cells than did APB MNCs. When incubated with RPMI‐1640 containing 10% fetal calf serum for 7 days, the percentage of CD45RA + cells within CD4 + T cells (%CD45RA + /CD4 + ) significantly decreased compared to that of fresh CB MNCs. IL‐15 exerted a dose‐dependent increase of %CD45RA + /CD4 + and a corresponding decrease of %CD45RO + /CD4 + in CB MNCs, an effect not observed with APB MNCs treated with IL‐15. The percentages of CD45RA + and CD45RO + expression within CD8 + cells, however, were not influenced by IL‐15, in either CB or APB MNCs. A greater number of CB MNCs underwent apoptosis than did APB MNCs after 7 days of culture in RPMI‐1640 containing 10% fetal calf serum. IL‐15 did not inhibit apoptosis but induced proliferation comparable to that achieved in APB MNCs. The ability of IL‐15 to preferentially enhance the proliferation of CD4 + CD45RA + cells in CB MNCs suggests a role for immunomodulative therapy in HIV‐infected newborns and infants.