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Reduced P‐selectin expression on circulating platelets after prolonged cold preservation in renal transplantation
Author(s) -
Scheinichen Dirk,
Vilsendorf Andreas Meyer zu,
Weißig Annette,
Bornscheuer Albrecht,
Becker Thomas,
Juettner Bjoern,
Mahr KarlHeinz,
Heine Joern
Publication year - 2003
Publication title -
clinical transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 76
eISSN - 1399-0012
pISSN - 0902-0063
DOI - 10.1034/j.1399-0012.2003.00073.x
Subject(s) - medicine , platelet , p selectin , transplantation , granulocyte , platelet activation , kidney , kidney transplantation , flow cytometry , immunology , andrology
The uremic state in patients with terminal renal insufficiency is accompanied by a bleeding tendency connected with platelet dysfunction. Prolonged cold ischemia and inflammatory interactions between leukocytes, platelets and endothelial cells contribute to ischemia‐/reperfusion (I/R) injury and may impair long‐term graft survival. We evaluated the influence of the duration of cold preservation time on the expression of platelet GPIIb/IIIa and P‐selectin and on the formation of leukocyte‐platelet complexes after kidney transplantation. Fourteen patients undergoing kidney transplantation were divided into group I with long preservation time (26.6 ± 1.9 h) and group II with short preservation time (8 ± 6.1 h). Five venous blood samples (3 ml) were taken before induction of anesthesia, 12 h, 2, 7 and 14 d after transplantation. Surface expression of the GPIIb/IIIa, P‐selectin and the percentage of platelet‐granulocyte complexes were quantified by flow cytometry. Additionally blood from seven healthy volunteers was analyzed. GPIIb/IIIa and P‐selectin expression on circulating platelets were significantly decreased in the long and the short‐term graft preservation group compared with healthy volunteers. A significantly reduced P‐selectin expression was found in the long‐term preservation group compared with the short‐term group. The percentage of platelet‐granulocyte complexes also decreased in both preservation groups in the first 2 d after reperfusion and remained in this state in the long‐term preservation group. Reduced expression of P‐selectin on circulating platelets may be an indicator of I/R injury after prolonged kidney graft preservation.