Premium
Differential diagnosis of kidney transplant rejection and cyclosporin/tacrolimus nephropathy using urine cytology
Author(s) -
Kyo Masahiro,
Ichikawa Yasuji,
Toki Kiyohide,
Nishimura Kennichi,
Fukunishi Takanobu,
Nagano Shunsuke,
Namba Yukiomi,
Gudat Fred,
Dalquen Peter,
Mihatsch M J
Publication year - 2002
Publication title -
clinical transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 76
eISSN - 1399-0012
pISSN - 0902-0063
DOI - 10.1034/j.1399-0012.16.s8.8.x
Subject(s) - medicine , papanicolaou stain , tacrolimus , urine cytology , urine , kidney , peripheral blood mononuclear cell , urology , creatinine , pathology , transplantation , kidney transplantation , cytology , urinary system , biology , biochemistry , cervical cancer , cystoscopy , cancer , in vitro
A total of 9000 urine samples from 69 kidney transplant recipients were studied for differential diagnoses of transplant rejection and cyclosporin/tacrolimus toxicity. New–Sternheimer and Papanicolaou staining were used to differentiate cells in urine. We also employed an immunocytochemical technique for further identification of exfoliated cells. With New–Sternheimer and Papanicolaou staining, the predominance of proximal tubular cells was useful to differentiate cyclosporin/tacrolimus toxicity from acute rejection in cases of increased serum creatinine level. During rejection episodes, an increased number of mononuclear cells and renal epithelial cells were found. Immunocytochemical analysis showed a significant increase of CD2‐, CD4‐ CD8‐, CD25‐ and HLA‐DR‐positive cells with rejection. However, there was no relationship between Banff criteria rejection grade and the increase of mononuclear cells.