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Effect of vitamin E on chromosomal aberrations in lymphocytes from patients with Down’s syndrome
Author(s) -
Pincheira J,
Navarrete MH,
De La Torre C,
Tapia G,
Santos MJ
Publication year - 1999
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1034/j.1399-0004.1999.550307.x
Subject(s) - basal (medicine) , lymphocyte , vitamin e , medicine , biology , vitamin , endocrinology , andrology , immunology , antioxidant , biochemistry , insulin
A possible protective effect of vitamin E ( dl ‐ α ‐tocoferol) on chromosomal damage was evaluated in lymphocytes from patients with Down’s syndrome (DS) and from controls. This included the analysis of the basal and G 2 chromosomal aberration frequencies in lymphocytes cultured with and without 100 μM vitamin E. The chromosomal damage in G 2 was determined by scoring the number of chromosomal aberrations in lymphocyte cultures treated with 5 mM caffeine, 2 h before harvesting.Vitamin E treatment decreased the basal and G 2 chromosomal aberrations both in control and DS lymphocytes. In DS cells, this protective effect, expressed as a decrease in the chromosomal damage, was greater (50%) than in controls (30%). These results suggest that the increment in basal and G 2 aberrations yield in DS lymphocytes may be related to the increase in oxidative damage reported in these patients.