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Analysis of the CD4 + T cell responses to house dust mite allergoid
Author(s) -
Kalinski P.,
Lebre M. C.,
Kramer D.,
De Jong E. C.,
Van Schijndel J. W. P. M.,
Kapsenberg M. L.
Publication year - 2003
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1034/j.1398-9995.2003.00240.x
Subject(s) - house dust mite , immunology , allergen , t cell , pyroglyphidae , allergy , polyclonal antibodies , cytokine , immunoglobulin e , medicine , biology , microbiology and biotechnology , immune system , antigen , antibody
Background: Modified allergen extracts (allergoids) with reduced IgE‐binding capacity are successfully used in immunotherapy of atopic allergy. Their reduced T‐cell stimulatory capacity is less well studied and is a subject of the present study. Methods: We compared the ability of native house dust mite extract ( Dermatophagoides pteronyssinus ; HDM) and the glutaraldehyde‐modified allergoid (HDM‐GA) to induce the proliferation and cytokine production by fresh PBMC and by DC‐stimulated polyclonal Th cells and HDM‐specific Th cell clones. Results: Freshly isolated T cells showed a partially reduced responsiveness to HDM‐GA, differentially pronounced in different donors. HDM‐specific Th cell clones prepared from three donors showed either a complete loss of reactivity to HDM‐GA, or completely preserved responsiveness. The frequency of nonreactive clones was donor‐dependent (2/3, 3/10 and 1/10). GA modification of HDM did not interfere with the cytokine production profile of HDM‐specific T cell clones. Conclusions: The reduced stimulatory potential of HDM‐GA results mainly from a loss of certain Th cell epitopes, rather than impaired allergen uptake and presentation, or induction of suppressive factors. Varying frequencies of allergoid‐nonreactive HDM‐specific Th cells may result in differential responses of individual patients to immunotherapy.