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Use of CD63 expression as a marker of in vitro basophil activation and leukotriene determination in metamizol allergic patients
Author(s) -
Gamboa P. M.,
Sanz M. L.,
Caballero M. R.,
Antépara I.,
Urrutia I.,
Jáuregui I.,
González G.,
Diéguez I.,
De Weck A. L.
Publication year - 2003
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1034/j.1398-9995.2003.00096.x
Subject(s) - basophil activation , medicine , allergy , cd63 , basophil , likelihood ratios in diagnostic testing , gastroenterology , immunology , allergen , immunoglobulin e , confidence interval , antibody , chemistry , microrna , biochemistry , microvesicles , gene
Background: We assessed the reliability of basophil activation test (FAST) and sulphidoleukotriene production (CAST) in the in vitro diagnosis of allergy to metamizol, evaluating its sensitivity and specificity. Methods: Twenty‐six patients allergic to metamizol and 30 control individuals were studied. Skin tests with metamizol, FAST, and CAST were performed. Results: FAST sensitivity was 42.3% and specificity 100%. The PPV of FAST is 100% and the NPV 99.4%. The likelihood ratio for a positive value cannot be calculated because the specificity is 100% and the likelihood ratio for a negative value is 0.58. CAST sensitivity was 52%, and specificity 90%. The PPV of the test is 5% and the NPV 99.5%. The likelihood ratio for a positive result was 5.2 and that for a negative result 0.53. FAST detects a larger number of cases when patients are studied within the first 6 months after the clinical reaction ( χ = 4.2, P = 0.04) than later. Together with skin tests, FAST allowed detection of 69.2% patients allergic to metamizol, the same as CAST 76%. The joint use of the three techniques allowed identification of 76.9% of cases. Conclusions: FAST and CAST are useful for the diagnosis of allergy to pyrazolones. Its usefulness clearly increases when recent reactions are studied.