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Localization of Der f 2 in the gut and fecal pellets of Dermatophagoides farinae
Author(s) -
Jeong K. Y.,
Lee I.Y.,
Ree H.I.,
Hong C.S.,
Yong T.S.
Publication year - 2002
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1034/j.1398-9995.2002.23623.x
Subject(s) - feces , midgut , mite , allergen , pellets , monoclonal antibody , house dust mite , biology , chemistry , allergy , microbiology and biotechnology , immunology , antibody , ecology , larva , paleontology
Background: House dust mite derived materials are known to be the most potent agent inducing allergic diseases. Localization of Der f 2 was attempted to specify the sites and concentrations of Der f 2 within the mite, which may indicate the importance of secreted materials and nonexcreted body components as allergen sources. Methods: Serial cryostat sections of embedded live mites and the fecal pellets, collected by brush, were immunoprobed using monoclonal antibody (mAb) 2F38 raised against recombinant (r) Der f 2. Results: Highest concentrations were found in the anterior midgut, implying that this is the site of Der f 2 synthesis and secretion. Digestive material and defecated fecal pellets were also labeled with mAb. Conclusions: Our results show that the major allergen, Der f 2, found in the house dust miteD. farinae is derived from the digestive tract, and is concentrated in the feces.