Premium
Measurement of IgE antibodies against purified grass‐pollen allergens (Phl p 1, 2, 3, 4, 5, 6, 7, 11, and 12) in sera of patients allergic to grass pollen
Author(s) -
Rossi R. E.,
Monasterolo G.,
Monasterolo S.
Publication year - 2001
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1034/j.1398-9995.2001.00258.x
Subject(s) - allergen , immunology , medicine , allergy , sensitization , immunoglobulin e , antibody
Background: Current allergy diagnosis is performed with allergen extracts which contain a variety of allergenic and nonallergenic components. The availability of highly purified and well‐characterized allergen molecules seems to be an advantage of component‐based diagnosis. Methods: With the immunoenzymatic CAP FEIA System, we measured specific IgE levels to the recombinant allergens rPhl p 1, rPhl p 2, rPhl p 5, rPhl p 6, rPhl p 7, rPhl p 11, rPhl p 12, and native Phl p 4 in 77 sera of patients allergic to grass pollen, in order to evaluate the IgE‐binding frequency to these purified grass‐pollen allergens and their relationship to rBet v 4, rBet v 2, and other allergens. Results: The frequency of sensitization was as follows: rPhl p 1=93.5%; rPhl p 2=67.5%; rPhl p 5=72.7%; rPhl p 6=68.8%; rPhl p 7=7.8%; rPhl p 11=53.2%; rPhl p 12=35.1%; and native Phl p 4=88.3%. As expected, rPhl p 7 and rPhl p 12 had a very good correlation (Spearman's r ) with Bet v 4 ( r =0.95%, P <0.05) and rBet v 2 ( r =0.99, P <0.05), respectively. Good correlations of rPhl p 12 with papain ( r =0.93, P <0.05), latex ( r =0.92, P <0.05), and bromelain ( r =0.86, P <0.05) were found. Highly variable individual sensitization patterns were observed. Conclusions: A new clinical approach has allowed the determination of specific allergograms for the different patients and may therefore be of great importance for more specific diagnosis. The use of component‐resolved diagnostics may be useful to evaluate the allergen content of an extract for immunotherapy by monitoring patient's IgE and IgG directed to relevant allergens.