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Selective upregulation of a functional β 7 integrin on differentiating eosinophils
Author(s) -
Lundahl J.,
Sehmi R.,
Hayes L.,
Howie K.,
Denburg J. A.
Publication year - 2000
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1034/j.1398-9995.2000.00574.x
Subject(s) - integrin , downregulation and upregulation , fibronectin , cell adhesion molecule , eosinophil , clone (java method) , microbiology and biotechnology , immunology , integrin alpha m , biology , cell adhesion , chemistry , cell , flow cytometry , biochemistry , extracellular matrix , dna , asthma , gene
Background: The sequence of adhesion‐molecule expression during eosinophil differentiation remains unclear. Methods: We analyzed the surface expression of α 4 , β 1 , and β 7 integrins and compared it to established myeloid developmental markers, using the eosinophilic cell line HL‐60 clone 15, as well as cord and peripheral blood differentiation assays. Results: Cells induced to eosinophil differentiation by treatment with butyric acid, IL‐5, and GM‐CSF showed a significant upregulation of β 7 integrin expression coincident with a marked upregulation of CD35 and attenuation of CD33 and β 1 integrin expression. In addition, adhesion of induced HL‐60 clone 15 cells to fibronectin was attenuated by a β 7 integrin antibody. Conclusions: Our data show that protein synthesis‐dependent upregulation of the functional β 7 integrin occurs under conditions when α 4 and β 1 integrins are fully expressed, indicating a sequential appearance of specific adhesion molecules on differentiating eosinophil progenitors.