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2S methionine‐rich protein (SSA) from sunflower seed isan IgE‐binding protein*[Note 1. * Published as Journal Series No.12741, Agricultural Research Division, ...]
Author(s) -
Kelly J. D.,
Hefle S. L.
Publication year - 2000
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1034/j.1398-9995.2000.00498.x
Subject(s) - sunflower , helianthus annuus , sunflower seed , methionine , immunoglobulin e , amino acid , allergen , biochemistry , allergy , biology , immunology , horticulture , antibody
Background: Sunflower seed contains 2S albumins that in other crops have been associated with allergenicity. The sunflower seed methionine‐rich 2S albumin (SSA) may be an IgE‐binding protein responsible for anaphylactic reactions in some sunflower seed‐sensitive subjects. The objective was to demonstrate that SSA is an IgE‐binding protein. Methods: SSA was purified and the amino‐acid sequence determined. The degree of purity of SSA was evaluated by silver staining, and its IgE‐binding capacity by immunoblotting with serum from a subject with a convincing clinical history of anaphylaxis to sunflower seed. Results: The amino‐acid sequence confirmed that the purified protein was the mature form of the methionine‐rich storage protein SSA from sunflower seed ( Helianthus annuus ). The SSA was specifically recognized by IgE from the serum of the sunflower seed‐allergic subject. Conclusions: SSA is an IgE‐binding protein, and subjects allergic to sunflower seed whose IgE binds to SSA are at risk of developing allergic reactions if they consume SSA.