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A 17‐kDa allergen detected in pine nuts
Author(s) -
GarcíaMenaya J. M.,
GonzaloGarijo M. A.,
Moneo I.,
Fernández B.,
GarcíaGonzález F.,
Moreno F.
Publication year - 2000
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1034/j.1398-9995.2000.00291.x
Subject(s) - immunoglobulin e , allergen , allergy , medicine , anaphylaxis , immunology , food allergens , food science , chemistry , antibody
Background: Few cases of allergy to pine nuts have been described. We report a case of anaphylactic reaction to pine nuts. The patient needed to be treated in the emergency room due to a systemic reaction immediately after eating pine nuts. Methods: The patient was studied by prick tests and prick by prick tests. Specific IgE was measured by CAP and by SDS–PAGE/immunoblotting by a diffusion method. Results: The patient showed positive prick by prick tests to pine nuts (12 mm of maximum wheal diameter). Specific IgE was positive (0.79 kU/l). The patient's serum recognized several proteins by immunoblot. However, a 17‐kDa allergen band was detected with high intensity. This protein was found to be sensitive to reducing agents, losing its IgE‐binding properties after reduction. Conclusions: The patient presented an IgE‐mediated reaction and detected a 17‐kDa protein from pine nuts not previously described.