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Interleukin‐8 inhalation directly provokes bronchoconstriction in guinea pigs
Author(s) -
Fujimura M,
Myou S,
Nomura M,
Mizuguchi M,
Matsuda T,
Harada A,
Mukaida N,
Matsushima K,
Harada A,
omura A
Publication year - 1999
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1034/j.1398-9995.1999.00891.x
Subject(s) - bronchoconstriction , inhalation , bronchoalveolar lavage , medicine , histamine , methacholine , anesthesia , terfenadine , immunology , asthma , pharmacology , respiratory disease , lung
Background: Although it has been reported that the concentration of interleukin (IL)‐8 in nasal lavage fluid and sputum and its production in bronchial epithelium were increased in asthmatic subjects, the direct effects of IL‐8 on the airways in vivo is unclear. Methods: We examined bronchoconstriction in response to IL‐8 inhalation through an endotracheal cannula in anesthetized, artifically ventilated guinea pigs. Results: Inhalation of IL‐8 at concentrations of 1 and 10 μg/ml caused significant bronchoconstriction, as revealed by the elevation of pressure at the airway opening. Moreover, the bronchoconstriction induced by IL‐8 was significantly inhibited by the antihistamines diphenhydramine and terfenadine, suggesting the involvement of histamine release in the IL‐8‐induced bronchoconstriction. No significant leukocyte infiltration was observed in the bronchoalveolar lavage fluid or histologic findings 25 min after the first IL‐8 inhalation. Conclusions: IL‐8 provokes bronchoconstriction without leukocyte accumulation in the airways, mediated in part by histamine release, in guinea pigs.