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Suppressive activity of bronchial macrophages recovered by induced sputum
Author(s) -
Fireman E,
Onn A,
Levo Y,
Bugolovov E,
Kivity S
Publication year - 1999
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1034/j.1398-9995.1999.00864.x
Subject(s) - sputum , pathogenesis , asthma , methacholine , immunology , medicine , cytokine , interleukin 4 , interleukin , interleukin 5 , respiratory disease , pathology , lung , tuberculosis
Background: The immunomodulatory activity of macrophages was shown to be a crucial mechanism in the pathogenesis of asthma. Methods: Induced sputum (IS) and methacholine challenge (MC) were carried out in 21 atopic subjects. Suppressive activity (SA) of sputum macrophages (SMO) was investigated on autologous peripheral lymphocytes (APL) proliferation in 12 of these patients and compared to the MC. Results: In 10 of the 21 patients, the FEV 1 was >80%; five of these had a nonreactive MC. Eosinophils and metachromatic cells correlated well ( r =0.6442; P =0.0029), but not with the MC. The SA of SMO correlated ( P =0.0152) with the MC: SMO enhanced APL proliferation in five patients with a positive MC, while SMO showed SA in five with a negative MC. Only two patients with suppressive SMO had a positive MC. Cytokine profiles from five patients showed that two patients with a negative MC had interleukin (IL)‐1α and β, IL‐6, and transforming growth factor (TGF)‐β transcripts, while two patients with a positive MC transcripted IL‐4 and IL‐5. One patient with a borderline MC transcripted IL‐5, but not IL‐4. Conclusions: These data support the theory that patients with reduced suppressive bronchial macrophages display clinical bronchial hyperreactivity.