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Latex allergy: characterization of major allergens and isotypic expression
Author(s) -
Chardin H,
Mayer C,
Desvaux Fx,
Sénéchal H,
Peltre G,
Chardin H
Publication year - 1999
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1034/j.1398-9995.1999.00138.x
Subject(s) - latex allergy , immunoglobulin e , allergy , immunology , allergen , western blot , antibody , nitrocellulose , gel electrophoresis , medicine , chemistry , biology , microbiology and biotechnology , biochemistry , membrane , gene
Latex allergy is now known to be related to type I hypersensitivity. In latex products, several reports have identified at least 10 allergens. However, 56 polypeptides have been shown to react to IgE by two‐dimensional gel electrophoresis and immunoprints. All studies using immunoprints have analyzed a relatively small number of sera. The aim of the present study was to analyze the specificity and the isotypic expression of a wide sample of latex‐sensitive patients. Latex proteins were separated by SDS–PAGE in an 8–18% gel gradient, transferred onto a nitrocellulose sheet by Western blot, and revealed by serum from latex‐sensitive patients. The presence of IgE, IgA, IgG1, IgG2, IgG3, and IgG4 was analyzed for each of the 54 sera. The results showed Hev b 3 to be the most frequently recognized protein, followed by Hev b 1, Hev b 7, and molecules of 94 kDa and 6–8 kDa. The most common isotypic association was IgE‐IgG1, frequently with IgA. IgG4 was also present in a little less than half of the IgE‐positive sera. IgG3 was also expressed but frequently recognized nonallergenic molecules. IgG2 was present in only two cases. Thus, as several authors have proposed a protective role for IgG in the allergic reaction, it may be of interest to compare these results with the course of the disease.