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Artefactual pyruvate and 2‐oxobutyrate produced by trimethylsilylation of methylmalonic and ethylmalonic acids in the presence of oxygen
Author(s) -
Mamer O. A.,
Choiniere L.,
Boismenu D.,
Lepine F.
Publication year - 1999
Publication title -
journal of inherited metabolic disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 102
eISSN - 1573-2665
pISSN - 0141-8955
DOI - 10.1023/a:1005510224963
Subject(s) - methylmalonic acid , chemistry , oxygen , pyruvic acid , trimethylsilyl , biochemistry , organic chemistry , homocysteine
Trimethylsilylation of methylmalonic and ethylmalonic acids in the presence of headspace atmospheric oxygen is shown to produce the trimethylsilyl derivatives of pyruvic and 2‐oxobutyric acids, along with 2‐hydroxy‐2‐methylmalonic and 2‐hydroxy‐2‐ethylmalonic acids, respectively. This may lead to overestimation of these keto acids, if they were not oximated in the original sample, and the mistaken reporting of the 2‐hydroxymalonates.