Open AccessDirect Analysis of Methylated Trivalent Arsenicals in Mouse Liver by Hydride Generation-Cryotrapping-Atomic Absorption Spectrometry
Author(s) -
Jenna M. Currier,
Milan Svoboda,
Diogo Pompéu de Moraes,
Tomáš Matoušek,
Jiřı́ Dědina,
Miroslav Stýblo
Publication year - 2011
Publication title -
chemical research in toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 156
eISSN - 1520-5010
pISSN - 0893-228X
DOI - 10.1021/tx200060c
Subject(s) - chemistry , inorganic arsenic , arsenic , hydride , limiting , detection limit , atomic absorption spectroscopy , mass spectrometry , absorption (acoustics) , extraction (chemistry) , inorganic chemistry , chromatography , metal , organic chemistry , mechanical engineering , physics , quantum mechanics , engineering , acoustics
Growing evidence suggest that the methylated trivalent metabolites of inorganic arsenic (iAs), methylarsonite (MAs(III)) and dimethylarsinite (DMAs(III)), contribute to adverse effects of iAs exposure. However, the lack of suitable methods has hindered the quantitative analysis of MAs(III) and DMAs(III) in complex biological matrices. Here, we show that hydride generation-cryotrapping-atomic absorption spectrometry can quantify both MAs(III) and DMAs(III) in livers of mice exposed to iAs. No sample extraction is required, thus limiting MAs(III) or DMAs(III) oxidation prior to analysis. The limits of detection are below 6 ng As/g of tissue, making this method suitable even for studies examining low exposures to iAs.