Open AccessIsotope Labeling-Based Quantitative Proteomics of Developing Seeds of Castor Oil Seed (Ricinus communis L.)
Author(s) -
Fábio César Sousa Nogueira,
Giuseppe Palmisano,
Veit Schwämmle,
Emanuela L Soares,
Arlete Aparecida Soares,
Peter Roepstorff,
Gilberto B. Domont,
Francisco Campos
Publication year - 2013
Publication title -
journal of proteome research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.644
H-Index - 161
eISSN - 1535-3907
pISSN - 1535-3893
DOI - 10.1021/pr400685z
Subject(s) - ricinus , isobaric labeling , castor oil , proteomics , quantitative proteomics , chemistry , chromatography , ricin , proteome , biochemistry , orbitrap , fractionation , fatty acid , catabolism , biology , botany , metabolism , mass spectrometry , gene , toxin
In this study, we used a mass spectrometry-based quantification approach employing isotopic (ICPL) and isobaric (iTRAQ) labeling to investigate the pattern of protein deposition during castor oil seed (Ricinus communis L.) development, including that of proteins involved in fatty acid metabolism, seed-storage proteins (SSPs), toxins, and allergens. Additionally, we have used off-line hydrophilic interaction chromatography (HILIC) as a step of peptide fractionation preceding the reverse-phase nanoLC coupled to a LTQ Orbitrap. We were able to identify a total of 1875 proteins, and from these 1748 could be mapped to extant castor gene models, considerably expanding the number of proteins so far identified from developing castor seeds. Cluster validation and statistical analysis resulted in 975 protein trend patterns and the relative abundance of 618 proteins. The results presented in this work give important insights into certain aspects of the biology of castor oil seed development such as carbon flow, anabolism, and catabolism of fatty acid and the pattern of deposition of SSPs, toxins, and allergens such as ricin and 2S albumins. We also found, for the first time, some genes of SSP that are differentially expressed during seed development.