Picomolar Detection of Protease Using Peptide/Single Walled Carbon Nanotube/Gold Nanoparticle-Modified Electrode | Zendy

Khaled A. Mahmoud | Zendy; Sabahudin Hrapovic | Zendy; John H. T. Luong | Zendy

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Picomolar Detection of Protease Using Peptide/Single Walled Carbon Nanotube/Gold Nanoparticle-Modified Electrode

Author(s) -

Khaled A. Mahmoud,

Sabahudin Hrapovic,

John H. T. Luong

Publication year - 2008

Publication title -

acs nano

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.554

H-Index - 382

eISSN - 1936-086X

pISSN - 1936-0851

DOI - 10.1021/nn8000774

Subject(s) - materials science , carbon nanotube , cyclic voltammetry , colloidal gold , electrode , dielectric spectroscopy , raman spectroscopy , nanoparticle , detection limit , ferrocene , nanotechnology , electrochemistry , analytical chemistry (journal) , chemistry , organic chemistry , chromatography , physics , optics

Picomolar electrochemical detection of human immunodeficiency virus type-1 protease (HIV-1 PR) using ferrocene (Fc)-pepstatin-modified surfaces has been presented. Gold electrode surface was modified with gold nanoparticles (AuNP) or thiolated single walled carbon nanotubes/gold nanoparticles (SWCNT/AuNP). Thiol-terminated Fc-pepstatin was then self-assembled on such surfaces as confirmed by Raman spectroscopy and scanning electron microscope. The interaction between the Fc-pepstatin-modified substrates and HIV-1 PR was studied by cyclic voltammetry and electrochemical impedance spectroscopy. Both electrode materials showed enhanced electrochemical responses to increasing concentrations of HIV-1 PR with shifting to higher potentials as well as decrease in the overall signal intensity. However, the sensing electrode modified with thiolated SWCNTs/AuNPs showed remarkable detection sensitivity with an estimated detection limit of 0.8 pM.

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