Spherical Nucleic Acids as a Divergent Platform for Synthesizing RNA–Nanoparticle Conjugates through Enzymatic Ligation | Zendy

Jessica L. Rouge | Zendy; Liang Hao | Zendy; Xiaochen A. Wu | Zendy; William E. Briley | Zendy; Chad A. Mirkin | Zendy

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Spherical Nucleic Acids as a Divergent Platform for Synthesizing RNA–Nanoparticle Conjugates through Enzymatic Ligation

Author(s) -

Jessica L. Rouge,

Liang Hao,

Xiaochen A. Wu,

William E. Briley,

Chad A. Mirkin

Publication year - 2014

Publication title -

acs nano

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.554

H-Index - 382

eISSN - 1936-086X

pISSN - 1936-0851

DOI - 10.1021/nn503601s

Subject(s) - nucleic acid , rna , dna , nanoparticle , conjugate , oligonucleotide , nanotechnology , sequence (biology) , chemistry , combinatorial chemistry , biophysics , computational biology , biochemistry , materials science , biology , gene , mathematical analysis , mathematics

Herein, we describe a rapid, divergent method for using spherical nucleic acids (SNAs) as a universal platform for attaching RNA to DNA-modified nanoparticles using enzyme-mediated techniques. This approach provides a sequence-specific method for the covalent attachment of one or more in vitro transcribed RNAs to a universal SNA scaffold, regardless of RNA sequence. The RNA-nanoparticle constructs are shown to effectively knock down two different gene targets using a single, dual-ligated nanoparticle construct.

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