Poly(2-deoxy-2-methacrylamido glucopyranose)-b-Poly(methacrylate amine)s: Optimization of Diblock Glycopolycations for Nucleic Acid Delivery | Zendy

Haibo Li | Zendy; Mallory A. Cortez | Zendy; Haley Phillips | Zendy; Yaoying Wu | Zendy; Theresa M. Reineke | Zendy

Open Access

Poly(2-deoxy-2-methacrylamido glucopyranose)-b-Poly(methacrylate amine)s: Optimization of Diblock Glycopolycations for Nucleic Acid Delivery

Author(s) -

Haibo Li,

Mallory A. Cortez,

Haley Phillips,

Yaoying Wu,

Theresa M. Reineke

Publication year - 2013

Publication title -

acs macro letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.966

H-Index - 92

ISSN - 2161-1653

DOI - 10.1021/mz300660t

Subject(s) - polynucleotide , cationic polymerization , nucleic acid , methacrylate , gene delivery , polymer chemistry , polymer , amine gas treating , transfection , chemistry , tertiary amine , copolymer , dynamic light scattering , materials science , biochemistry , organic chemistry , nanoparticle , nanotechnology , gene

A series of nine poly(2-deoxy-2-methacrylamido glucopyranose)- b -poly(methacrylate amine) diblock copolycations The cationic block was varied in length and in the degree of methyl group substitution (secondary, tertiary, quaternary) on the pendant amine in an effort to optimize the structure and activity for plasmid DNA delivery. Upon a thorough kinetic study of polymerization for each polymer, the glycopolymers were prepared with well-controlled M n and Ð. The binding and colloidal stability of the polymer-pDNA nanocomplexes at different N/P ratios and in biological media has been investigated using gel electrophoresis and light scattering techniques. The toxicity and transfection efficiency of the polyplexes has been evaluated with Hep G2 (human liver hepatocellular carcinoma) cells; several polymers displayed excellent delivery and toxicity profiles justifying their further development for in vivo gene therapy.

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