Perfluorophenyl Azide Immobilization Chemistry for Single Molecule Force Spectroscopy of the Concanavalin A/Mannose Interaction | Zendy

Carolin Madwar | Zendy; William Chu Kwan | Zendy; Lingquan Deng | Zendy; Olof Ramström | Zendy; Rolf Schmidt | Zendy; Shan Zou | Zendy; Louis A. Cuccia | Zendy

Open Access

Perfluorophenyl Azide Immobilization Chemistry for Single Molecule Force Spectroscopy of the Concanavalin A/Mannose Interaction

Author(s) -

Carolin Madwar,

William Chu Kwan,

Lingquan Deng,

Olof Ramström,

Rolf Schmidt,

Shan Zou,

Louis A. Cuccia

Publication year - 2010

Publication title -

langmuir

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.042

H-Index - 333

eISSN - 1520-5827

pISSN - 0743-7463

DOI - 10.1021/la1036579

Subject(s) - chemistry , concanavalin a , mannose , azide , molecule , force spectroscopy , spectroscopy , organic chemistry , photochemistry , computational chemistry , polymer chemistry , biochemistry , physics , quantum mechanics , in vitro

The versatility of perfluorophenyl azide (PFPA) derivatives makes them useful for attaching a wide variety of biomolecules and polymers to surfaces. Herein, a single molecule force spectroscopy (SMFS) study of the concanavalin A/mannose interaction was carried out using PFPA immobilization chemistry. SMFS of the concanavalin A/mannose interaction yielded an average unbinding force of 70-80 pN for loading rates between 8000 and 40,000 pN/s for mannose surfaces on aminated glass, and an unbinding force of 57 ± 20 pN at 6960 pN/s for mannose surfaces on gold-coated glass. Dynamic force spectroscopy was used to determine the dissociation rate constant, k(off), for this interaction to be 0.16 s(-1).

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