Open AccessSynthesis and Structure–Activity Relationship Studies of Novel Dihydropyridones as Androgen Receptor Modulators
Author(s) -
Antonella Pepe,
Michael Pamment,
Yeong Sang Kim,
Sunmin Lee,
Min-Jung Lee,
Kristin Beebe,
Anton V. Filikov,
Len Neckers,
Jane B. Trepel,
Sanjay V. Malhotra
Publication year - 2013
Publication title -
journal of medicinal chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.01
H-Index - 261
eISSN - 1520-4804
pISSN - 0022-2623
DOI - 10.1021/jm301714s
Subject(s) - antiandrogens , lncap , chemistry , androgen receptor , homology modeling , antagonism , structure–activity relationship , prostate cancer , antagonist , antiandrogen , molecular model , stereochemistry , computational biology , receptor , pharmacology , androgen , medicine , biochemistry , in vitro , cancer , hormone , biology , enzyme
A library of 3-hydroxy-2,3-dihydropyridones was synthesized, and their activities as antiandrogens were tested in the human prostate cancer cell line LNCaP. Structure-activity relationship (SAR) studies resulted in the identification of a potent compound whose activity is comparable to that of MDV3100. Homology modeling and molecular mechanics were used to build a structural model of the androgen receptor-ligand binding domain and to investigate the structural basis of the antagonism. The model is qualitatively consistent with the observed SAR. Moreover, the enrichment plot shows that screening with the model performs significantly better than random screening. Therefore, the model probably represents a realistic conformation of the antagonist form and can be utilized for structure-based design of novel antiandrogens.