Boron Doped Diamond Biosensor for Detection of Escherichia coli | Zendy

Ehsan Majid | Zendy; Keith B. Male | Zendy; John H. T. Luong | Zendy

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Boron Doped Diamond Biosensor for Detection of Escherichia coli

Author(s) -

Ehsan Majid,

Keith B. Male,

John H. T. Luong

Publication year - 2008

Publication title -

journal of agricultural and food chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.203

H-Index - 297

eISSN - 1520-5118

pISSN - 0021-8561

DOI - 10.1021/jf8013958

Subject(s) - escherichia coli , detection limit , chemistry , chromatography , tetramer , sodium dodecyl sulfate , biosensor , boron , tap water , enzyme , nuclear chemistry , biochemistry , organic chemistry , environmental engineering , engineering , gene

o-Nitrophenol, released from o-nitrophenyl-beta-D-galactopyranose as catalyzed by beta-galactosidase, a tetramer of Escherichia coli, has been exploited for the detection of E. coli contamination in foodstuffs. This reaction was detected using a boron doped diamond electrode poised at +0.93 V, without any surface modification. The enzyme was effectively induced by isopropyl-beta-D-thiogalacto-pyranoside with the maximum enzyme activity observed with sodium dodecyl sulfate at 50 degrees C. A biphasic calibration plot was observed: 4 x 10(4) to 2 x 10(5) cells/mL and 2 x 10(5) to 6 x 10(6) cells/mL for the first and second region, respectively. The detection limit was 4 x 10(4) cells/mL with a total analysis time of <1.5 h. Electrode fouling was easily overcome by approximately 40 rapid CV scans, and the method was applicable for detecting E. coli in artificially spiked samples of beef, pork, chicken, milk, and tap water.

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