Simple and Validated Quantitative 1H NMR Method for the Determination of Methylation, Acetylation, and Feruloylation Degree of Pectin | Zendy

Judith Müller-Maatsch | Zendy; Augusta Caligiani | Zendy; Tullia Tedeschi | Zendy; Kathy Elst | Zendy; Stefano Sforza | Zendy

Open Access

Simple and Validated Quantitative ¹H NMR Method for the Determination of Methylation, Acetylation, and Feruloylation Degree of Pectin

Author(s) -

Judith Müller-Maatsch,

Augusta Caligiani,

Tullia Tedeschi,

Kathy Elst,

Stefano Sforza

Publication year - 2014

Publication title -

journal of agricultural and food chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.203

H-Index - 297

eISSN - 1520-5118

pISSN - 0021-8561

DOI - 10.1021/jf502679s

Subject(s) - pectin , chemistry , repeatability , chromatography , acetylation , titration , methanol , hydrolysis , methylation , proton nmr , reproducibility , quantitative analysis (chemistry) , ferulic acid , acetic anhydride , organic chemistry , biochemistry , gene , catalysis

The knowledge of pectin esterification degree is of primary importance to predict gelling and other properties of pectin from different sources. This paper reports the development of a simple and rapid (1)H NMR-based method for the simultaneous quantitative determination of methylation, acetylation, and feruloylation degree of pectin isolated from various food sources. Pectin esters are hydrolyzed in NaOH/D2O, and the obtained methanol, acetic acid, and ferulic acid are directly measured by (1)H NMR. High accuracy, repeatability, and reproducibility of the method were obtained, and the analysis time is reduced as compared to conventional chromatography- or titration-based methods.

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