Open AccessMethods for Cryosectioning and Mass Spectrometry Imaging of Whole-Body Zebrafish
Author(s) -
Whitney L. Stutts,
Megan Knuth,
Måns Ekelöf,
Debabrata Mahapatra,
Seth W. Kullman,
David C. Muddiman
Publication year - 2020
Publication title -
journal of the american society for mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.961
H-Index - 127
eISSN - 1879-1123
pISSN - 1044-0305
DOI - 10.1021/jasms.9b00097
Subject(s) - mass spectrometry imaging , danio , zebrafish , chemistry , mass spectrometry , desorption electrospray ionization , maldi imaging , matrix assisted laser desorption/ionization , chromatography , biochemistry , desorption , ion , ionization , chemical ionization , organic chemistry , gene , adsorption
The zebrafish ( Danio rerio ) is an ideal model for whole animal studies of lipid metabolism and lipid-related disease. In this work, infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) was applied for direct visualization of lipid and metabolite distributions across various organs in whole-body zebrafish tissue sections. Detailed methods for overcoming the challenges of cryosectioning adult male zebrafish for MSI and complementary histological imaging are described. Representative two-dimensional ion maps demonstrated organ specific localization of lipid analytes allowing for visualization of areas of interest including the brain, liver, intestines, and skeletal muscle. A high resolving power mass spectrometer was utilized for accurate mass measurements, which permitted the use of open-source, web-based tools for MS 1 annotations including METASPACE and METLIN. Whole-body MSI with IR-MALDESI allowed for broad lipid coverage with high spatial resolution, illustrating the potential of this technique for studying lipid-related diseases using zebrafish as a model organism.