Ambient Single-Cell Analysis and Native Tissue Imaging Using Laser-Ablation Electrospray Ionization Mass Spectrometry with Increased Spatial Resolution | Zendy

Michael J. Taylor | Zendy; Andrey Liyu | Zendy; Ákos Vértes | Zendy; Christopher Anderton | Zendy

Open Access

Ambient Single-Cell Analysis and Native Tissue Imaging Using Laser-Ablation Electrospray Ionization Mass Spectrometry with Increased Spatial Resolution

Author(s) -

Michael J. Taylor,

Andrey Liyu,

Ákos Vértes,

Christopher Anderton

Publication year - 2021

Publication title -

journal of the american society for mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.961

H-Index - 127

eISSN - 1879-1123

pISSN - 1044-0305

DOI - 10.1021/jasms.1c00149

Subject(s) - chemistry , mass spectrometry , mass spectrometry imaging , analytical chemistry (journal) , resolution (logic) , electrospray ionization , laser ablation , laser , ambient ionization , ionization , desorption electrospray ionization , maldi imaging , metabolomics , image resolution , optics , chromatography , chemical ionization , ion , matrix assisted laser desorption/ionization , artificial intelligence , physics , organic chemistry , adsorption , desorption , computer science

Laser-ablation electrospray ionization mass spectrometry (LAESI-MS) is an emerging method that has the potential to transform the field of metabolomics. This is in part due to LAESI-MS being an ambient ionization method that requires minimal sample preparation and uses (endogenous) water for in situ analysis. This application note details the employment of the "LAESI microscope" source to perform spatially resolved MS analysis of cells and MS imaging (MSI) of tissues at high spatial resolution. This source configuration utilizes a long-working-distance reflective objective that permits both visualization of the sample and a smaller LAESI laser beam profile than conventional LAESI setups. Here, we analyzed 200 single cells of Allium cepa (red onion) and imaged Fittonia argyroneura (nerve plant) in high spatial resolution using this source coupled to a Fourier transform mass spectrometer for high-mass-resolution and high-mass-accuracy metabolomics.

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