Open AccessA Versatile Platform for Mass Spectrometry Imaging of Arbitrary Spatial Patterns
Author(s) -
Kenneth P. Garrard,
Måns Ekelöf,
Sitora Khodjaniyazova,
M. Caleb Bagley,
David C. Muddiman
Publication year - 2020
Publication title -
journal of the american society for mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.961
H-Index - 127
eISSN - 1879-1123
pISSN - 1044-0305
DOI - 10.1021/jasms.0c00128
Subject(s) - mass spectrometry imaging , sample (material) , mass spectrometry , computer vision , pixel , rectangle , data acquisition , artificial intelligence , region of interest , computer graphics (images) , imaging spectrometer , computer science , chemistry , spectrometer , optics , physics , mathematics , chromatography , geometry , operating system
A vision-system driven platform, RastirX, has been constructed for mass spectrometry imaging (MSI) of arbitrary two-dimensional patterns. The user identifies a region of interest (ROI) by drawing on a live video image of the sample with the computer mouse. Motion commands are automatically generated to move the sample to acquire scan data for the pixels in the ROI. Synchronization of sample stage motion with laser firing and mass spectrometer (MS) scan acquisition is fully automated. RastirX saves a co-registered optical image and the scan location information needed to convert raw MS data into imzML format. Imaging an arbitrarily shaped ROI instead of the minimal enclosing rectangle reduces contamination from off-sample material and significantly reduces acquisition time.